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Structural Biology Facilities at the Department of Biochemistry

 

Cryo-EM Forum @ Biochemistry

In order to promote the use and expand our knowledge of Cryo-EM technique, we’ve created a Cryo-EM Forum @ Biochemistry – a series of informal seminars with invited experts in the area of Cryo-EM. Please subscribe to our Cryo-EM mailing list to receive future seminar announcements, Cryo-EM facility updates, and to engage in conversation about the exciting world of Cryo-EM.

Organising team: Andrzej Szewczak-Harris and Dima Chirgadze

Next Talk:

TBA


External links

For those who want to know more about cryo-electron microscopy, we would like to recommend the following resources.


Facility Instruments information

Titan Krios Falcon 3 (TEM mode):

  • magnification 75,000 – 1.06* Angstrom/pixel
  • magnification 96,000 – 0.85* Angstrom/pixel
  • magnification 120,000 – 0.66 Angstrom/pixel

Titan Krios K3 (EFTEM mode), super-resolution in brackets:

  • magnification 26,000 – 3.5 (1.7) Angstrom/pixel
  • magnification 33,000 – 2.8 (1.4) Angstrom/pixel
  • magnification 42,000 – 2.2 (1.1) Angstrom/pixel
  • magnification 53,000 – 1.8 (0.88) Angstrom/pixel
  • magnification 64,000 – 1.43 (0.71) Angstrom/pixel
  • magnification 81,000 – 1.07* (0.535) Angstrom/pixel
  • magnification 105,000 – 0.83* (0.415) Angstrom/pixel
  • magnification 130,000 – 0.65* (0.325) Angstrom/pixel
  • magnification 165,000 – 0.54 (0.27) Angstrom/pixel

Talos Arctica Falcon 3:

  • magnification 36,000 – 2.9 Angstrom/pixel
  • magnification 45,000 – 2.3 Angstrom/pixel
  • magnification 57,000 – 1.82 Angstrom/pixel
  • magnification 73,000 – 1.43* Angstrom/pixel
  • magnification 92,000 – 1.13* Angstrom/pixel
  • magnification 120,000 – 0.89 Angstrom/pixel
  • magnification 150,000 – 0.70 Angstrom/pixel

*pixel sizes were confirmed by collecting high-resolution EM data and comparing the obtained reconstruction to the corresponding high-resolution X-ray structure.