Biophysics resources

Surface Plasmon Resonance (SPR/Biacore)

BioLayer Interferometry (BLI/Octet)

ForteBio/Sartorius Octet platform
"Biolayer interferometry for measuring the kinetics of protein–protein interactions and nanobody binding" in Nature Protocols, 2025. doi.org/10.1038/s41596-024-01079-8

Isothermal Titration Calorimetry (ITC)

MicroCal range (Malvern)
NITPIC and GUSSI for integration and baseline correction based on SVD treatment of peak shapes, ready for SEDPHAT, see PMID: 22530732.

Circular Dichroism Spectrometry (CD)

Mass Photometry

Refeyn Ltd
PhotoMol analysis from the European Molecular Biology Laboratory (EMBL) Hamburg Station

Analytical Ultracentrifugation (AUC)

SEDFIT and SEDPHAT from the NIH with latest software downloads
GUSSI (for nice graphical output from SEDFIT and SEDPHAT)
Ultrascan
Nottingham Centre for Macromolecular Hydrodynamics
Excellent instructional video: “Assembly, Loading, and Alignment of an Analytical Ultracentrifuge Sample Cell” at the Journal of Visualized Experiments
SEDNTERP, available in various flavours, also here
What is the frictional ratio?
Calculate the frictional ratio from a pdb file using the HullRad server
Nanolytics Instruments (was Spin Analytical)
Chemical resistance tables (including epoxy/carbon centrepieces)
SVEDBERG and DCDT+ from John Philo

Dynamic Light Scattering (DLS)

Dynamic Scanning Fluorimetry (DSF, “Thermofluor”)

“The use of differential scanning fluorimetry to detect ligand interactions that promote protein stability” in Nature Protocols, 2007. PMID: 17853878
Web-based analysis tool for thermal shift assays

Stopped-flow

General Biophysics