Surface Plasmon Resonance (SPR/Biacore)
BioLayer Interferometry (BLI/Octet)
Isothermal Titration Calorimetry (ITC)
- MicroCal range (Malvern)
- NITPIC for integration and baseline correction based on SVD treatment of peak shapes, ready for SEDPHAT. PMID: 22530732.
- SCORPIO database of ITC data from structurally-characterised complexes
Circular Dichroism Spectrometry (CD)
- Dichroweb at Birkbeck, plus course notes
- Dicroprot
- DichroCalc
- K2D3
- Bestsel
- Electromagnetic waves and circular dichroism: an animated tutorial – fantastic visual explanation of the CD phenomenon by András Szilágyi
Mass Photometry
- Refeyn Ltd
- PhotoMol analysis from the European Molecular Biology Laboratory (EMBL) Hamburg Station
Analytical Ultracentrifugation (AUC)
- SEDFIT and SEDPHAT from the NIH with latest software downloads
- GUSSI (for nice graphical output from SEDFIT and SEDPHAT)
- Ultrascan
- Nottingham Centre for Macromolecular Hydrodynamics
- Excellent instructional video: “Assembly, Loading, and Alignment of an Analytical Ultracentrifuge Sample Cell” at the Journal of Visualized Experiments
- SEDNTERP, available in various flavours, also here
- What is the frictional ratio?
- Calculate the frictional ratio from a pdb file using the HullRad server
- Converting S to S20,w
- Spin Analytical
-
(including epoxy/carbon centrepieces)
- SVEDBERG and DCDT+ from John Philo
Dynamic Light Scattering (DLS)
- Malvern Zetasizer Nano S
- Zetasizer software download (free!)
-
(e.g. Z-average, polydispersity…)
- Minimum concentration considerations
-
(found within software, under “Tools”)
- Create custom reports (YouTube)
Dynamic Scanning Fluorimetry (DSF, “Thermofluor”)
- “The use of differential scanning fluorimetry to detect ligand interactions that promote protein stability” in Nature Protocols, 2007. PMID: 17853878
- Web-based analysis tool for thermal shift assays
Stopped-flow
General Biophysics
- ε205 calculator
-
by Joe Maman
- Hydrodynamic modelling software (HYDRO suite) from José García de la Torre’s lab
- Hydrodynamic parameters from bead models using US-SOMO
- Hydrodynamic parameters using the HullRad server
